4G8Y
Crystal structure of Ribonuclease A in complex with 5b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-21 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.301, 32.672, 72.380 |
| Unit cell angles | 90.00, 89.35, 90.00 |
Refinement procedure
| Resolution | 13.884 - 1.800 |
| R-factor | 0.2018 |
| Rwork | 0.199 |
| R-free | 0.25700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2g8q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.165 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 22087 | |
| <I/σ(I)> | 12.4 | 2.8 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 7.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 25% PEG 4000, 20 mM Nacitrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
