4G8X

G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	70
Detector technology	CCD
Collection date	2008-03-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0389
Spacegroup name	C 1 2 1
Unit cell lengths	219.137, 56.951, 57.030
Unit cell angles	90.00, 104.79, 90.00

Refinement procedure

Resolution	37.710 - 3.001
R-factor	0.2853
Rwork	0.281
R-free	0.32770
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.690
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.110
High resolution limit [Å]	3.000	3.000
Number of reflections	13818
Completeness [%]		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.16M Calcium acetate, 0.08 M Sodium Cacodylate pH 6.5, 15% PEG 8000, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.16M Calcium acetate, 0.08 M Sodium Cacodylate pH 6.5, 15% PEG 8000, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K