4G8G

Crystal Structure of C12C TCR-HA B2705-KK10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-16
Detector	ADSC QUANTUM 210
Wavelength(s)	0.956
Spacegroup name	P 1 21 1
Unit cell lengths	56.682, 70.320, 107.767
Unit cell angles	90.00, 98.77, 90.00

Refinement procedure

Resolution	46.729 - 2.400
R-factor	0.182
Rwork	0.178
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kgc 1ogt
RMSD bond length	0.008
RMSD bond angle	1.128
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.400
Number of reflections	32993
Completeness [%]	100.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	16% PEG 8000 and 0.1M MES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K