4G8D
Crystal structures of N-acyl homoserine lactonase AidH S102G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2011-07-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.426, 129.941, 44.387 |
| Unit cell angles | 90.00, 110.84, 90.00 |
Refinement procedure
| Resolution | 19.825 - 1.350 |
| R-factor | 0.1347 |
| Rwork | 0.133 |
| R-free | 0.16750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g5x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 99376 | |
| Completeness [%] | 50.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 30% PEG8000, 0.2M LiAc, 0.1M NaAc, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
