4G87

Crystal structure of GLMU from Mycobacterium tuberculosis snapshot 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-02-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	77.000, 77.000, 276.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.840 - 2.030
R-factor	0.179
Rwork	0.178
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	2.091
Data scaling software	XSCALE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.844	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.107	0.723
Number of reflections	38608
<I/σ(I)>	18.51	2.6
Completeness [%]	96.9	89.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	8% PEG 8000, 150MM NACL, 5% GLYCEROL, 1,3-BUTANEDIOL, AMPPNP, MGCL2, 0.1M HEPES, COCL2, DTT, pH 7.5, VAPOR DIFFUSION, temperature 277K