4G85
Crystal structure of human HisRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.449, 100.449, 257.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.110 |
| R-factor | 0.2738 |
| Rwork | 0.271 |
| R-free | 0.32730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hri |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.069 | 0.025 | 0.696 |
| Number of reflections | 24210 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 98.8 | 87.2 | 99.9 |
| Redundancy | 5.5 | 4.8 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2 M ammonium citrate 20% PEGMME 2000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
