4G83
Crystal Structure of p73 DNA-Binding Domain Tetramer bound to a Full Response-Element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 141.717, 96.345, 34.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.610 - 4.000 |
| R-factor | 0.2443 |
| Rwork | 0.241 |
| R-free | 0.30730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vd0 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 3.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 4.000 |
| Rmerge | 0.140 |
| Number of reflections | 5072 |
| <I/σ(I)> | 8.88 |
| Completeness [%] | 99.8 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M BTP, 20% PEG3350 , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
