4G7W
Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-13 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97922 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 126.250, 126.250, 128.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.990 - 2.900 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.26193 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.853 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.470 | 2.980 |
| High resolution limit [Å] | 2.900 | 3.350 | 2.900 |
| Number of reflections | 13171 | ||
| Completeness [%] | 99.6 | 100 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20 mM Bis Tris, 50 mM NaCl, Fos-Choline-9, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
