4G7T

Rat Heme Oxygenase-1 in complex with Heme and CO with 1 hr Illumination: Laser on

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-08
Detector	MAR CCD 165 mm
Wavelength(s)	1.033
Spacegroup name	P 32 2 1
Unit cell lengths	65.877, 65.877, 120.319
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.810 - 1.900
R-factor	0.17527
Rwork	0.173
R-free	0.21028
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4g7p
RMSD bond length	0.022
RMSD bond angle	2.926
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.6.0117)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	22725
<I/σ(I)>	15.8	3.4
Completeness [%]	92.7	95.4
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K