4G7O
Crystal structure of Thermus thermophilus transcription initiation complex containing 2 nt of RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 185.483, 103.883, 297.340 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 46.606 - 2.993 |
| R-factor | 0.2054 |
| Rwork | 0.205 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.993 | 2.993 |
| Rmerge | 0.174 | 0.870 |
| Number of reflections | 221194 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 98.4 | 91.2 |
| Redundancy | 11.4 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | 100 mM Tris-HCl, pH 8.7, 200 mM potassium chloride, 50 mM magnesium chloride, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
