4G6X
Crystal structure of glyoxalase/bleomycin resistance protein from Catenulispora acidiphila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 61.969, 61.969, 77.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.800 - 1.730 |
| R-factor | 0.16685 |
| Rwork | 0.166 |
| R-free | 0.19105 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.059 | 0.450 |
| Number of reflections | 30170 | |
| <I/σ(I)> | 42.24 | 1.8 |
| Completeness [%] | 99.1 | 87.1 |
| Redundancy | 7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCL, 1.5 M Ammonium Sulfate, 15% Glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
