4G6U
CdiA-CT/CdiI toxin and immunity complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.699, 103.631, 125.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.353 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.22870 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 22419 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 14.3 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1 M sodium acetate pH 5.5, 0.2 M NaCl. 18% PEG-6000, 10 mM yttrium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
