4G6Q
The crystal structure of a functionally unknown protein Kfla_6221 from Kribbella flavida DSM 17836
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.759, 77.759, 140.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.158 - 2.080 |
| R-factor | 0.1918 |
| Rwork | 0.189 |
| R-free | 0.23690 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.095 | 0.577 |
| Number of reflections | 26743 | |
| <I/σ(I)> | 49.3 | 5.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.7 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1M Tris:HCl, 1.5M Ammonium Phosphate Dibasic, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
