4G6O
Crystal Structure of the ERK2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.634, 70.262, 60.244 |
Unit cell angles | 90.00, 109.55, 90.00 |
Refinement procedure
Resolution | 29.870 - 2.200 |
R-factor | 0.1821 |
Rwork | 0.180 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | BUSTER-TNT (BUSTER 2.11.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 55.000 | 55.000 | 2.060 |
High resolution limit [Å] | 2.000 | 4.580 | 2.000 |
Rmerge | 0.041 | 0.025 | 0.203 |
Number of reflections | 23439 | ||
<I/σ(I)> | 18.1 | ||
Completeness [%] | 89.6 | 97.9 | 50.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES buffer, pH 6.5, 26-28% PEG-MME 2000, 200 mM ammonium sulfate and 20 mM 2-mercaptoethanol, vapor diffusion, temperature 298K |