4G6F
Crystal Structure of 10E8 Fab in Complex with an HIV-1 gp41 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-10-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.021, 71.432, 129.236 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 42.590 - 2.100 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.074 | 0.056 | 0.242 |
| Number of reflections | 61890 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 88.6 | 93.7 | 52.2 |
| Redundancy | 2.7 | 3.1 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 40% PEG 400, 0.1 M NaCitrate, 0.1 M Tris pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
