4G68
Biochemical and structural insights into xylan utilization by the thermophilic bacteriumcaldanaerobius polysaccharolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Collection date | 2010-06-01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.330, 150.862, 150.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.2313 |
| Rwork | 0.230 |
| R-free | 0.26050 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0056) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.094 | 0.071 | 0.242 |
| Number of reflections | 126799 | ||
| <I/σ(I)> | 11.8 | ||
| Completeness [%] | 99.8 | 99.8 | 98.4 |
| Redundancy | 7.9 | 7.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | PEG 1500, pH 7.5, vapor diffusion, hanging drop, temperature 282K |
