4G59
Crystal structure of the murine cytomegalovirus MHC-I homolog m152 with ligand RAE-1 gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-03-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.443, 99.800, 68.609 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 47.516 - 2.440 |
| R-factor | 0.2032 |
| Rwork | 0.201 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PBD entries 2O5N 1jfm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.070 | 0.080 |
| Number of reflections | 45215 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 94.7 | 96.4 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 15% PEG3350, 0.2M KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
