4G57
Staphylococcal Nuclease double mutant I72L, I92L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2011-04-08 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.200, 48.200, 63.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 1.810 |
| R-factor | 0.19242 |
| Rwork | 0.190 |
| R-free | 0.23224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.486 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MIFit8 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.220 | 24.120 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.310 | ||
| Number of reflections | 15345 | ||
| <I/σ(I)> | 37.3 | 0.8 | |
| Completeness [%] | 53.6 | ||
| Redundancy | 2.17 | 1.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 57% MPD, 200 mM Tris.HCl buffer, approx. 6 mg/ml protein, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
