4G4S

Structure of Proteasome-Pba1-Pba2 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Collection date	2011-03-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	C 2 2 21
Unit cell lengths	132.409, 182.513, 388.782
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.778 - 2.490
R-factor	0.1839
Rwork	0.183
R-free	0.23150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.146
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.490
Number of reflections	162817
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1 M sodium citrate, 18-20% PEG 3000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K