4G4P
Crystal structure of glutamine-binding protein from Enterococcus faecalis at 1.5 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0098 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.730, 61.130, 45.120 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 36.008 - 1.500 |
| R-factor | 0.1169 |
| Rwork | 0.115 |
| R-free | 0.15500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.205 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.033 | 0.105 |
| Number of reflections | 43037 | |
| <I/σ(I)> | 20.4 | 7.4 |
| Completeness [%] | 90.6 | 57.1 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 28% PEG 1500,100 mM MMT, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
