4G43
Structure of the chicken MHC class I molecule BF2*0401 complexed to P5E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-12-08 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.920, 40.241, 132.622 |
| Unit cell angles | 90.00, 120.35, 90.00 |
Refinement procedure
| Resolution | 41.306 - 1.803 |
| R-factor | 0.1849 |
| Rwork | 0.183 |
| R-free | 0.21790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.306 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.185 | |
| Number of reflections | 69202 | |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 75.0 | 75 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 5% MPD, 20% PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
