4G41
Crystal structure of s-adenosylhomocysteine nucleosidase from streptococcus pyogenes in complex with 5-methylthiotubericidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.735, 72.345, 116.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.470 - 1.450 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.19744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | S.AUREUS MTAN |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.314 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.620 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.033 | 0.247 |
| Number of reflections | 66215 | |
| <I/σ(I)> | 27.3 | 2.4 |
| Completeness [%] | 87.5 | 21.3 |
| Redundancy | 4.4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG MME 5000, 0.1M MES, 0.2M AMMONIUM SULPHATE, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
