4G3P

Crystal structure of GlmU from Mycobacterium tuberculosis Snapshot 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-02-09
Detector	MAR scanner 345 mm plate
Spacegroup name	H 3
Unit cell lengths	77.470, 77.470, 277.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.860 - 2.470
R-factor	0.184
Rwork	0.181
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dj4
RMSD bond length	0.022
RMSD bond angle	2.009
Data scaling software	XSCALE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.860	2.530
High resolution limit [Å]	2.470	2.470
Rmerge	0.123	0.488
Number of reflections	22217
<I/σ(I)>	16.4	3.45
Completeness [%]	99.3	92.8
Redundancy	6.3	5.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	8% PEG 8000, 150MM NACL, 5% GLYCEROL, 1,3-BUTANEDIOL,AMPPNP, MGCL2,0.1M HEPES, COCL2, DTT, PH 7.5, VAPOR DIFFUSION, TEMPERATURE 277K, vapor diffusion