4G3O
Crystal structure of the CUE domain of the E3 ubiquitin ligase AMFR (gp78)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9183 |
| Spacegroup name | P 65 |
| Unit cell lengths | 55.396, 55.396, 30.993 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.000 - 1.600 |
| R-factor | 0.16001 |
| Rwork | 0.159 |
| R-free | 0.18626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ejs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 6929 | |
| <I/σ(I)> | 55.2 | 8.1 |
| Completeness [%] | 1.0 | 100 |
| Redundancy | 11.1 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.056 M sodium phosphate, 1.344 M potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
