4G3N

Mycobacterium tuberculosis gyrase type IIA topoisomerase C-terminal domain at 1.4 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	38.700, 80.519, 88.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.000 - 1.400
R-factor	0.1868
Rwork	0.185
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zi0
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.090
High resolution limit [Å]	1.400
Number of reflections	53387
Completeness [%]	96.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES 100 mM PH 7.5, NaCl 200 mM, PEG 3350 20-30%, VAPOR DIFFUSION, HANGING DROP, temperature 293K