4G3I
Crystal structure of Dpo4 in complex with DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.472, 52.634, 99.445 |
| Unit cell angles | 75.40, 80.35, 70.97 |
Refinement procedure
| Resolution | 95.810 - 2.500 |
| R-factor | 0.24337 |
| Rwork | 0.241 |
| R-free | 0.29363 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2agq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.923 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.813 | 2.610 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 33414 | |
| <I/σ(I)> | 12.4 | 4.1 |
| Completeness [%] | 97.2 | 96.1 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.2 M CaCl2, 0.1 M Na Cacodylate pH 6.5, 19 % PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
