4G3H

Crystal structure of helicobacter pylori arginase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	94.690, 102.240, 148.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.900 - 2.200
R-factor	0.23
Rwork	0.218
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.258
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.918
High resolution limit [Å]	2.200	2.200
Number of reflections	66320
Completeness [%]	89.8	89.8
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	25% polyethylene glycol 3350, 100mM Bis-Tris pH 5.5, 1mM MnCl2, 15mM guanidine hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K