4G3C
Crystal structure of apo murine Nf-kappaB inducing kinase (NIK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97740 |
| Spacegroup name | P 43 |
| Unit cell lengths | 142.211, 142.211, 45.083 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.150 |
| R-factor | 0.18999 |
| Rwork | 0.186 |
| R-free | 0.22547 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | homology model |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 49462 | |
| <I/σ(I)> | 19.9 | 2.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 276 | 2uL protein with 2uL well solution of 0.5 M ammonium sulfate, 0.9 M lithium sulfate,0.1M sodium citrate tribasic dihydrate , pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 276K |
