4G3A

Crystal Structure of MAST/Orbit N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	36.180, 97.579, 149.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.789 - 1.994
R-factor	0.1897
Rwork	0.188
R-free	0.22860
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.014
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.000
High resolution limit [Å]	1.994
Rmerge	0.059
Number of reflections	36131
<I/σ(I)>	28.9
Completeness [%]	97.4
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	12.5% PEG 4000, 0.1M Bis-Tris, 0.1M NaCl & 20% v/v glycerol with a 1:1 ratio of protein to precipitant, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K