4G36
Photinus pyralis luciferase in the adenylate-forming conformation bound to DLSA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2006-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.040, 93.040, 297.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.664 - 2.624 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2d1s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.168 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_837)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.770 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.079 | 0.277 |
| Number of reflections | 45585 | |
| <I/σ(I)> | 15.6 | 5.9 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 300 mM Na/K Tartrate, 20% PEG 6000, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
