4G2V

Structure complex of LGN binding with FRMPD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 61 2 2
Unit cell lengths	93.835, 93.835, 172.863
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.234 - 2.400
R-factor	0.1916
Rwork	0.190
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.712
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rmerge	0.103
Number of reflections	18332
Completeness [%]	100.0
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M Sodium Chloride, 0.1M BISTIS PH6.5, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K