4G2D

Crystal structure of the hyperthermophilic Sulfolobus islandicus PLL SisLac

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.980
Spacegroup name	P 32 2 1
Unit cell lengths	47.810, 47.810, 239.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.410 - 2.700
R-factor	0.26612
Rwork	0.265
R-free	0.29244
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vc5
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.410
High resolution limit [Å]	2.700
Number of reflections	9436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2 M AMMONIUM SULFATE, 0.1 M TRIS, 0.2M LITHIUM SULFATE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293KK