4G26
Crystal Structure of proteinaceous RNase P 1 (PRORP1) from A. thaliana with Ca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.790, 111.883, 140.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.090 - 1.750 |
| R-factor | 0.16483 |
| Rwork | 0.162 |
| R-free | 0.20872 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g23 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.398 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 65735 | |
| <I/σ(I)> | 12.1 | 1.5 |
| Completeness [%] | 98.2 | 88.1 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 18% PEG 3,350, 0.1 M sodium citrate pH 5.5, and 0.02 M CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
