4G1U
X-ray structure of the bacterial heme transporter HmuUV from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.870, 101.230, 105.210 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 29.773 - 3.008 |
| R-factor | 0.2649 |
| Rwork | 0.263 |
| R-free | 0.29480 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.007 |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.070 | |
| High resolution limit [Å] | 2.990 | 13.390 | 2.990 |
| Rmerge | 0.067 | ||
| Rmeas | 0.031 | 1.297 | |
| Number of reflections | 42310 | 456 | 2801 |
| <I/σ(I)> | 11.71 | 35.94 | 1.28 |
| Completeness [%] | 98.8 | 89.1 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP |
