4G1T

Crystal structure of interferon-stimulated gene 54

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Collection date	2010-10-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	80.023, 95.207, 155.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.572 - 2.800
R-factor	0.2369
Rwork	0.234
R-free	0.28660
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.242
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Number of reflections	29706
Completeness [%]	99.0	98.8	87.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	PEG, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	PEG, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K