4G1P
Structural and Mechanistic Basis of Substrate Recognition by Novel Di-peptidase Dug1p From Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-16 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 119.132, 119.132, 176.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.731 - 2.547 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zof |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.135 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.547 | 2.550 |
| Rmerge | 0.216 | 0.874 |
| Number of reflections | 24507 | |
| <I/σ(I)> | 2.27 | |
| Redundancy | 11.3 | 11.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.5 M AmSO4, 0.1 M tri-Na citrate pH 5.6, 1.0 M Lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
