4G1J

Sortase C1 of GBS Pilus Island 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	P 1
Unit cell lengths	38.977, 48.467, 59.369
Unit cell angles	87.31, 76.85, 72.64

Refinement procedure

Resolution	46.250 - 1.750
R-factor	0.173
Rwork	0.172
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.250
High resolution limit [Å]	1.750
Number of reflections	31194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1