4G19
Crystal structure of the glutathione transferase GTE1 from Phanerochaete chrysosporium in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.573, 88.146, 156.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.892 - 2.000 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f03 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.029 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.480 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.375 |
| Number of reflections | 81606 | |
| <I/σ(I)> | 20 | 4.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 277 | 30% PEG 8000, 200 mM sodium acetate and 100 mM HEPES., pH 7.0, MICROBATCH, temperature 277K |
