4G0P

Crystal Structure of Arabidopsis thaliana AGO1 MID domain in complex with UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	200
Detector technology	CCD
Detector	RIGAKU SATURN 944+
Spacegroup name	P 21 21 21
Unit cell lengths	37.291, 60.842, 69.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.794 - 1.800
R-factor	0.1769
Rwork	0.175
R-free	0.21610
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.016
RMSD bond angle	1.362
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.5_2)
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.794	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	13152
Completeness [%]	89.5	78.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	0.1 M citric acid, 2 M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K