4G0M

Crystal structure of Arabidopsis thaliana AGO2 MID domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	200
Detector technology	CCD
Collection date	2010-01-27
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	67.647, 67.647, 223.222
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.640 - 2.306
R-factor	0.2087
Rwork	0.206
R-free	0.23720
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.077
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX (1.5_2)
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.640	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	21269
Completeness [%]	92.9	63.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	0.1 M citric acid, 2.0 M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K