4G08
Crystal structure of the periplasmic domain of InvG
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-12-07 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.978170, 0.979310, 0.979100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.270, 56.660, 74.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.086 - 1.801 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23080 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.051 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.256 |
| Number of reflections | 11517 | |
| <I/σ(I)> | 18.7 | 7.3 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 7.8 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Tris, pH 8.0, 30% Jeffamine M-600, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
