4G03
High-resolution Crystal Structural Variance Analysis between Recombinant and Wild-type Human Serum Albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.570, 58.670, 95.675 |
| Unit cell angles | 75.71, 88.07, 73.63 |
Refinement procedure
| Resolution | 47.219 - 2.216 |
| R-factor | 0.2382 |
| Rwork | 0.236 |
| R-free | 0.30140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ao6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.219 | 2.270 |
| High resolution limit [Å] | 2.216 | 2.230 |
| Rmerge | 0.069 | |
| Number of reflections | 58401 | |
| <I/σ(I)> | 23.337 | |
| Completeness [%] | 97.3 | 97.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 28-30% (w/v) PEG 3350, Sigma-Aldrich, 50mM potassium phosphate, pH 7.0, VAPOR DIFFUSION, temperature 291K |
