4FZV
Crystal structure of the human MTERF4:NSUN4:SAM ternary complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9790, 0.9795, 0.9500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 298.667, 53.226, 52.963 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 46.815 - 2.000 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19030 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.316 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.815 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 55979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.15 M magnesium formate, 50 mM Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
