4FZL
High resolution structure of truncated bacteriocin syringacin M from Pseudomonas syringae pv. tomato DC3000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97631 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.810, 96.090, 64.270 |
| Unit cell angles | 90.00, 114.17, 90.00 |
Refinement procedure
| Resolution | 50.920 - 1.460 |
| R-factor | 0.15406 |
| Rwork | 0.152 |
| R-free | 0.20117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.097 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.920 | 1.500 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.052 | 0.966 |
| Number of reflections | 106648 | |
| <I/σ(I)> | 13.6 | 2.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 8% w/v PEG 8000, 30% v/v ethylene glycol, 0.13 M CaCl2, 0.03 M MgCl2, 0.1 M Bicine/Tris base, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
