4FZH

Structure of the Ulster Strain Newcastle Disease Virus Hemagglutinin-Neuraminidase Reveals Auto-Inhibitory Interactions Associated with Low Virulence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77.2
Detector technology	CCD
Collection date	2011-02-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97916
Spacegroup name	P 31 2 1
Unit cell lengths	124.606, 124.606, 284.914
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.913 - 3.501
R-factor	0.2395
Rwork	0.236
R-free	0.30420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e8t
RMSD bond length	0.006
RMSD bond angle	1.040
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (for MR)
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.913
High resolution limit [Å]	3.500
Number of reflections	33126

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	291	17% PEG 8000, 200 mM MgCl2, 100 mM Tris, pH 7.5, EVAPORATION, temperature 291K