4FZE
Crystal structure of N26_i1 Fab, an ADCC mediating anti-HIV-1 antibody.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.636, 124.452, 41.433 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 41.088 - 1.999 |
| R-factor | 0.1913 |
| Rwork | 0.186 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d9l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.999 | 2.000 |
| Rmerge | 0.123 | 0.838 |
| Number of reflections | 32042 | |
| <I/σ(I)> | 8.9 | 1.1 |
| Completeness [%] | 98.8 | 86 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate pH 6.5, and 30% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
