4FZD
Crystal structure of MST4-MO25 complex with WSF motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 239.259, 239.259, 239.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.250 |
| R-factor | 0.2219 |
| Rwork | 0.220 |
| R-free | 0.26013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1UPL and 3GGF |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.133 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 18633 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.1M Tris pH 8.0, 18% PEG 350 mme, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
