4FZ8
Crystal structure of C11 Fab, an ADCC mediating anti-HIV-1 antibody.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-07 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.547, 56.011, 70.708 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 34.350 - 2.660 |
| R-factor | 0.2558 |
| Rwork | 0.253 |
| R-free | 0.31260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tnn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.660 | 2.700 |
| Rmerge | 0.146 | 0.752 |
| Number of reflections | 14023 | |
| <I/σ(I)> | 16.1 | 1.3 |
| Completeness [%] | 97.3 | 87.3 |
| Redundancy | 5.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 0.14 M sodium citrate, 0.07 M HEPES sodium pH 7.5, 14% isopropanol, and 30% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
