4FZ5

Crystal Structure of Human TIRAP TIR-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0809
Spacegroup name	P 43 2 2
Unit cell lengths	100.253, 100.253, 78.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.600
Rwork	0.324
R-free	0.34600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y92
RMSD bond length	0.035
RMSD bond angle	3.152
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.600
Number of reflections	4941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	5%(v/v) PEG200, 0.1M TRIS, pH8.5, EVAPORATION, temperature 298K