4FYS
Human aminopeptidase N (CD13) in complex with angiotensin IV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 64 |
Unit cell lengths | 157.907, 157.907, 115.327 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.540 - 2.010 |
R-factor | 0.16997 |
Rwork | 0.169 |
R-free | 0.19791 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.421 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
Rmerge | 0.121 | 0.054 | 0.348 |
Number of reflections | 103185 | ||
<I/σ(I)> | 11.7 | ||
Completeness [%] | 96.4 | 90.6 | 99 |
Redundancy | 7.4 | 7.5 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop vapor diffusion | 5 | 295 | 2M ammonium sulfate, 0.1M sodium acetate, 10% glycerol, pH 5.0, hanging drop vapor diffusion, temperature 295K |